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KMID : 0381120200420010013
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Genes and Genomics
2020 Volume.42 No. 1 p.13 ~ p.24
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Identification of source-sink tissues in the leaf of Chinese cabbage (Brassica rapa ssp. pekinensis) by carbohydrate content and transcriptomic analysis
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Lee Jeong-Yeo
Dong Xiangshu
Choi Kwan
Song Ha-Yong
Yi Han-Kuil
Hur Yoon-Kang
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Abstract
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Background: A leaf of Chinese cabbage (Brassica rapa ssp. pekinensis) is composed of a photosynthetic blade and a non-photosynthetic large midrib; thus each leaf contains both source and sink tissues. This structure suggests that, unlike in other plants, source-sink metabolism is present in a single leaf of Chinese cabbage.
Objective: This study was designed to identify the transport route of photosynthetic carbon and to determine whether both source and sink tissues were present in a leaf.
Methods: Plant samples were collected diurnally. Their carbohydrate contents were measured, and a genome-wide transcriptome analysis was performed using the Br300K microarray. Expression profiles of selected genes were validated using qRT-PCR analysis.
Results: The presence of two contrasting tissues (blade as source and midrib as sink) in a leaf was demonstrated by (1) diurnal distribution patterns of starch and sucrose content; (2) Gene Ontology (GO) enrichment analysis of microarray data; (3) expression profiles of photosynthetic and sucrose biosynthetic genes; and (4) expression patterns of a variety of sugar transporter genes.
Conclusion: Source and sink tissues were both present in Chinese cabbage leaves, but the midrib functioned as a sink tissue as well as a site exporting to roots and other sink tissues. Function of most genes discriminating between source and sink tissue appeared to be regulated largely at the post-transcriptional level, not at the transcriptional level.
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KEYWORD
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Carbohydrate content, Br300K microarray, Large midrib, Photosynthetic genes, Sugar transporters
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